Journal: Molecular Oncology

Article Title: Disruption of tp53 leads to cutaneous nevus and melanoma formation in Xenopus tropicalis

doi: 10.1002/1878-0261.13301

Figure Lengend Snippet: Histology‐based nevus and melanoma classification is confirmed by the expression levels of molecular marker genes. (A–F) Representative morphology of dissected skin samples under stereo microscope. For each category, two frogs were biopsied and similar morphology was observed. (A) Ventral skin sample from a 19‐month‐old wild‐type frog; (B) ventral skin sample from a 19‐month‐old tp53 Δ7/Δ7 frog without nevus/melanoma lesions; (C–E) ventral skin samples from 19‐month‐old tp53 Δ7/Δ7 frogs with benign nevus, dysplastic nevus, and melanoma in situ , respectively; (F) ventral skin sample from a 25‐month‐old tp53 Δ7/Δ7 frog with an invasive melanoma. Scale bars, 100 μm. (G–L) Representative histological structures (H&E staining) of samples shown in A–F, respectively. Similar structures were observed in 10 serial sections from each sample. Scale bars, 50 μm. (M) RT‐PCR analyses revealed the transcriptional expression levels of genes indicated on the right side in different samples listed on the top. gapdh was used as an RNA loading control. Identical results were obtained in two independent RT‐PCR experiments.

Article Snippet: Each dissected lesion was first photographed using a stereo microscope (SMZ18; Nikon, Tokyo, Japan) (Fig. ) and then divided into two halves, one again for histological documentation (H&E staining) (Fig. ) and the other half for RNA extraction and RT‐PCR analysis (Fig. ).

Techniques: Expressing, Marker, Microscopy, In Situ, Staining, Reverse Transcription Polymerase Chain Reaction, Control